Description
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for HB-beta has been pre-coated onto a microplate. Add HB-beta (Standards or samples) to the well, and then add Biotin-conjugated HB-beta. A competitive inhibition reaction is launched between HB-beta (Standards or samples) and Biotin conjugated HB-beta with the HB-beta antibody. Then add the HRP-avidin to each well. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of HB-beta bound in the initial step. The color development is stopped and the intensity of the color is measured